Would perfusing my mice with 4% PFA after injecting with NaF affect my results?

More Alaa Ousta's questions See All

Could anyone recommend software for drawing the physical structure of nanoparticles?

I am looking for professional software to draw the physical structure of nanoparticles or vesicles, not the chemical structures for which we use ChemDraw, but the actual physical structures,...

23 June 2024 1,292 3 View

How could I analyze the results of caspase-glo 3/7 assay?

I have done caspase-glo 3/7 assay which measures caspase activity after cleavage and generation of luminescent signal produced by luciferase. However,after I got the results, I am not sure how to...

02 June 2024 7,833 2 View

How can I identify an unknown gene of known activity ?

Hi, I've observed a new biological activity in certain cells, likely induced by an enzyme. How can I determine the gene or genes responsible for expressing this unknown enzyme? Thanks in advance

31 May 2024 9,111 3 View

Hello everyone, I am looking for collaboration to do clinical research?

I have many skills like data analysis with R programming or Spss , scientific writing and paper publishing

23 May 2024 1,683 3 View

Does GFP interferes with absorbance in MTT assay or luminescence in Caspase 3/7 Glo assay?

I have cells that produce GFP as a control, and I intend to perform the MTT and Caspase 3/7 Glo assays. The emission wavelength of GFP is 508 nm, and its absorption is 475 nm. In addition, I will...

16 April 2024 3,537 2 View

I would like to know why I obtained such false positive curves, which appears on both linear ang log scales healthy curves?

The positive curves for E coli different strains from mucual stool sample.

29 March 2024 5,907 0 View

Methylation of spata 6 gene in rat males?

methylation of spata 6 gene and its role on male infertility of rat

08 March 2024 9,667 0 View

How to prepare concentration MPs for field scale ( phytoremediation of floating plants ( water lily, water mimosa, water hyacinth )?

how to prepare concentration MPs for field scale ( phytoremediation of floating plants ( water lily, water mimosa, water hyacinth )?

04 March 2024 1,822 0 View

How to choose the suitable labtop?

I am a researcher who decided to buy labtop suitable for my work as a simulation and data analysis work Please suggest one

23 February 2024 3,635 2 View

What is the maximum duration for storage of isolated extracellular vesicles from serum in -80 for the purpose of protein content assessment?

Note: Serum isolated extracellular vesicles using immunoprecipitation (magnetic beads)

20 February 2024 4,894 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

Difficulty with permittivitt and Magnetic Permeability Calculations?

Difficulty with permittivitt and Magnetic Permeability Calculations Hello everyone, I have all the parameters related to the calculations of the permittivitty and magnetic permeability...

30 July 2024 5,206 1 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

Subhash C. Juneja

In my guess, it should not affect your results. PFA fix everything where it reaches instantly

Alaa Ousta

Thank you, Subhash C. Juneja !

Valeria Tananska

Alaa hello,

Only if applied under certain conditions. Also, there are other factors involved. Please, read here:

Article Transient blood–brain barrier disruption is induced by low p...