Fluorojade-C troubleshooting experience?

Hello all, I've tried a Fluorojade-C stain according to the manufacturer's recommendations on FFPE tissue sections. My problem is that there is no staining, not even the DAPI control. I prepared...

11 January 2021 9,749 0 View

RNA extraction from bacteria culture ?

Hi everyone, I am currently working on RNA extraction from bacterial (gram-neg) cultures for whole-transcritpome sequencing. I use a protocol as follow: Bacterial cell pellets are resuspended in...

11 November 2020 4,842 4 View

Where can I purchase an inducible episomal expression vector with FP reporter casette?

Hello, I am looking for an inducible episomal expression vector system which works in mammalian hematopoietic cells. The best scenario would be a bidirectional system, but an IRES-containing...

24 February 2020 7,970 1 View

Regression with one dummy-coded IV not significant?

Hi together, I am looking for an answer to an SPSS related question, using only ONE dummy coded IV with two groups. Other than that my model only includes moderators and control variables. After...

23 September 2019 2,522 5 View

Transform variables to stationarity - Growth rate or differences?

I am running a VAR with a single stock price and a sentiment measure as endogenous variables, and the DAX, the IMX (real estate index), industry turnover, wages and unemployment as exogenous...

01 September 2019 2,548 12 View

Which interrater agreement measure is most suitable for complex coding in qualitative research?

Dear peers, I am currently working on a very large qualitative project. We have 46 interviews and a very complex coding scheme: 2 topics, 6 sub-topics each with each sub-topic having about 3-4...

15 January 2019 2,837 6 View

How can I increase the expression of murine trypsinogen 7 in 293T cells?

Hey, I have a pcDNA 3.1 plasmid with a murine trypsinogen 7 sequence. I tried various transfection methods (lipofectamine2000, FuGene, Electroporation) into HEK 293T cells, but I had very low...

17 September 2018 9,932 2 View

How to normalize enhancer RNA expression from total RNA-Seq?

hello, I like to quantify expression changes at enhancers of a specific TF and later correlate them to gene expression changes (same data set). I have the positions of the enhancers from ChIP-Seq...

07 February 2018 2,521 8 View

Can I do a PCA on variables which are not measured from the same subjects?

I have a dataset in which ~800 subjects participated in rating questionnaire items in response to visual stimuli. The problem is however, that each individual subject only rated the images on a...

22 May 2017 4,112 12 View

How to measure transfer of training (typical behavior on the job)?

26 April 2017 8,735 5 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View