Would it be scientifically acceptable to compare a seasonal pollen index of two years with a long year average PI?

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Can I use HistoGel to embed fragile Tissues?

Hello everyone, I am working with Precision Cut liver slices of 250 µm. These need to be processed for FFPE. As you can imagenie they are very fragile and tend to break when I embedd them in...

16 November 2021 2,690 3 View

How to report degrees of freedom of Welch's t-test according to APA 7?

For most test statistics degrees of freedom are integers. For Welch's t-Test however, degrees of freedom are often decimals. According to APA 7, should I report the exact degrees of freedom or...

16 April 2021 5,802 1 View

Fluorojade-C troubleshooting experience?

Hello all, I've tried a Fluorojade-C stain according to the manufacturer's recommendations on FFPE tissue sections. My problem is that there is no staining, not even the DAPI control. I prepared...

11 January 2021 9,993 0 View

Which Primers work well as negative control in H3K27Ac CHIP-qPCR?

We aim to verify ATAC Seq data by CHIP-qPCR of the ROIs and are currently establishing all the controls. We use anti-H3K27me3 and H3K27Ac to pull down our histone marks of interest. We also used...

06 January 2021 6,662 0 View

RNA extraction from bacteria culture ?

Hi everyone, I am currently working on RNA extraction from bacterial (gram-neg) cultures for whole-transcritpome sequencing. I use a protocol as follow: Bacterial cell pellets are resuspended in...

11 November 2020 5,040 4 View

Where can I purchase an inducible episomal expression vector with FP reporter casette?

Hello, I am looking for an inducible episomal expression vector system which works in mammalian hematopoietic cells. The best scenario would be a bidirectional system, but an IRES-containing...

24 February 2020 8,116 1 View

Regression with one dummy-coded IV not significant?

Hi together, I am looking for an answer to an SPSS related question, using only ONE dummy coded IV with two groups. Other than that my model only includes moderators and control variables. After...

23 September 2019 2,667 5 View

Transform variables to stationarity - Growth rate or differences?

I am running a VAR with a single stock price and a sentiment measure as endogenous variables, and the DAX, the IMX (real estate index), industry turnover, wages and unemployment as exogenous...

01 September 2019 2,748 12 View

Cohort follow-up with adult participants, should I be using adult measures or child measures?

I will be following up a cohort of participants who were last interviewed 10 years ago. At the time we used validated measure for all sorts of health, educational and social outcomes suitable for...

10 July 2019 9,506 11 View

MPLUS: Centered items in TYPE = COMPLEX possible?

Hi, I'm analyzing a CFA in MPLUS. To consider the multilevel structure of the data I'm using TYPE=COMPLEX. My supervisor told me to calculate the group-mean-centering of the items (before using...

17 June 2019 4,346 3 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View