RNA extraction from bacteria culture ?

Hi everyone, I am currently working on RNA extraction from bacterial (gram-neg) cultures for whole-transcritpome sequencing. I use a protocol as follow: Bacterial cell pellets are resuspended in...

11 November 2020 4,842 4 View

Where can I purchase an inducible episomal expression vector with FP reporter casette?

Hello, I am looking for an inducible episomal expression vector system which works in mammalian hematopoietic cells. The best scenario would be a bidirectional system, but an IRES-containing...

24 February 2020 7,970 1 View

Regression with one dummy-coded IV not significant?

Hi together, I am looking for an answer to an SPSS related question, using only ONE dummy coded IV with two groups. Other than that my model only includes moderators and control variables. After...

23 September 2019 2,522 5 View

Transform variables to stationarity - Growth rate or differences?

I am running a VAR with a single stock price and a sentiment measure as endogenous variables, and the DAX, the IMX (real estate index), industry turnover, wages and unemployment as exogenous...

01 September 2019 2,548 12 View

Which interrater agreement measure is most suitable for complex coding in qualitative research?

Dear peers, I am currently working on a very large qualitative project. We have 46 interviews and a very complex coding scheme: 2 topics, 6 sub-topics each with each sub-topic having about 3-4...

15 January 2019 2,837 6 View

How can I increase the expression of murine trypsinogen 7 in 293T cells?

Hey, I have a pcDNA 3.1 plasmid with a murine trypsinogen 7 sequence. I tried various transfection methods (lipofectamine2000, FuGene, Electroporation) into HEK 293T cells, but I had very low...

17 September 2018 9,932 2 View

How to normalize enhancer RNA expression from total RNA-Seq?

hello, I like to quantify expression changes at enhancers of a specific TF and later correlate them to gene expression changes (same data set). I have the positions of the enhancers from ChIP-Seq...

07 February 2018 2,521 8 View

Can I do a PCA on variables which are not measured from the same subjects?

I have a dataset in which ~800 subjects participated in rating questionnaire items in response to visual stimuli. The problem is however, that each individual subject only rated the images on a...

22 May 2017 4,112 12 View

How to measure transfer of training (typical behavior on the job)?

26 April 2017 8,735 5 View

I am looking for an ecological footprint calculator for individuals. Can anyone recommend a questionnaire?

The study aims to identify the main obstacles that prevent people from acting more environmental-friendly and therefore reducing their individual footprint.

15 January 2017 1,555 12 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View