Hello,
I am looking for an inducible episomal expression vector system which works in mammalian hematopoietic cells. The best scenario would be a bidirectional system, but an IRES-containing bicistronic one would also be ok. It should, of course, have an antibiotic resistance like AMP for bacteria-based amplification as well. Could anyone recommend/provide a system with a manageable cloning site and a RFP or GFP casette?
Thank you for your help!
Franziska
Dear Dr. Briest,
non-constitutive but inducible systems:
1. https://systembio.com/products/gene-expression-systems/eev-systems/
https://systembio.com/shop/cumate-inducible-cuo-eev-cloning-and-expression-vector/
2. https://www.addgene.org/browse/article/6787/
https://www.addgene.org/collections/bacterial-expression/
3. https://www.jenabioscience.com/lexsy-expression/lexsy-configurations/inducible-episomal LEXSY
4. https://www.biocat.com/genomics/cloning-kits/enhanced-episomal-vectors-eev-technology-non-integrative-sustained-expression
5. https://en.vectorbuilder.com/learning-center/vector-system/pRP_Tet_On.html
Best of Luck!
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