Would I expect to see lowered CD14+ expression from a population of total (unsorted) monocytes that are dying?

01 January 2017 1 8K Report

Cells in culture for 72h.

Hi Sharon,

The expression of CD14 on monocytes is a marker for their functional status as you can read in this core paper on monocyte nomenclature. http://www.bloodjournal.org/content/116/16/e74.long?sso-checked=true (if the link does'nt work use the doi: 10.1182/blood-2010-02-258558)

As you can read in the paper, as they turn into non-classical monocytes (sometimes also named "inflammatory") the expression of CD14 is down-regulated and they start evidencing their effector functions (phagocytosis, production of cytokines, etc). Therefore using the reduction of CD14-expression as a marker for apoptosis is not correct. My suggestion is that you use some other stainings to determine apoptosis as an Annexin V/PI staining, or any of the many available viability dyes.

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