I am interested in evaluating antioxidant properties, but, I have a huge extract library. So, I want to do a first screening assay with DPPH, using only one concentration (the same for all of them) to define the best ones. Which will be the ideal concentration range to do the assay?
It is important to take into account that they are extracts, no pure compounds. So, I don't want to underestimate the metabolites that are minorities in the extracts.
Thank you for sharing your opinion.
To take 50 µL of crude microbial extracts for DPPH assay shows a good results.
Thank you Karthik Raju for you reply. But, if you are going to do the assay with one unique concentration (the same for all extracts), which will be the best option? For example, 100 ug/mL or 1 mg/mL.
Dear Jeysson sir, unique concentration is rise some question while go for publication, so you can choose three different type of concentrations like 50,75,100 ug/mL or 100,200,300 ug/mL (for all the extracts) which one shows a better percentage of inhibition is your expectation.
Dear Jeysson,
I consider that you should choose an average concentration with respect to the concentrations normally tested in most of the studies that you are consulting. If I am correctly understanding your question, you know neither the compounds responsible for the antioxidant activity nor the concentrations of these in each extract. Therefore, although you do not want to underestimate the metabolites that are in low proportion in the extract, you have no other option. I believe that the screening with a single concentration can allow you to find the best extracts and eventually, evaluate if there are synergies or antagonisms in the extracts with better performance and that surely you plan to elucidate in the future. I hope I could have helped you. Regards,
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