Chelex vs. Glassmilk extraction approach for DNA sequencing?

Hi, I am looking into methods to extract DNA from fish tissue. Our lab has used the glassmilk approach in the past but recently switched to using Chelex. I was wondering if anyone knew of any...

24 September 2019 7,467 1 View

Is it possible to find the average of responses to a self-report scale?

My participants have rated themselves 1-10 on a series of 19 statements. Those statements are supposed to represent 5 different constructs. So for example, questions 2, 6 and 11 are all about...

06 February 2019 3,473 2 View

How long can PCR products labelled with FAM dye be stored before it begins to degrade?

I have PCR products stored at -20 degrees (Celsius) that are tagged with FAM dye. How long can these be kept before the fluorescent signal diminishes?

01 December 2018 2,081 3 View

How to calculate protein interaction energy using VMD NAMD2?

I am using the VMD plugin QwikMD to do MD simulations of my protein-protein complexes. Hereafter I am using the NAMD2 analysis to calculate my total energy of the system along with all of the...

11 May 2018 1,481 5 View

Liquid culture colony PCR technique?

I need to do a pcr on a liquid culture of E.coli. I'm amplifying a 150bp region of gyrA, using primers that I designed. I'm using NEB Next Ultra 2 Q5 MM. For the reaction, I am using 5ul NEB, 0.5...

25 October 2017 5,995 5 View

I would like to store 10 uL aliquots of bacterial culture. What would be the best way?

I need to store 10ul aliquots of bacterial culture , would the best way be to put them in PCR tubes with some gylcerol (10ul 50%) at -80?

31 January 2017 2,848 3 View

What distinguishes the fourth industrial revolution from previous radical economic development eras ?

04 March 2016 6,069 11 View

How do you prevent holes from forming in air-liquid airway epithelial cell culture after removal of apical media?

02 September 2015 9,185 1 View

Some people claim that a person can have too much antioxidants, preventing for instance the proper useful functionality of free radicals?

But maybe they are just propagandists. Might you have any information or thoughts on that?

01 January 1970 5,107 4 View

When drivers are looking at sat navs, do the distractions lead to more accidents?

Can you measure the time drivers would be looking at the sat navs and in congested urban areas would this be significant?

01 January 1970 7,179 2 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

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01 March 2021 3,905 5 View

How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

01 March 2021 3,325 4 View

Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What are the important tips for writing review articles?

Important tips for writing a good review articles

24 February 2021 8,544 6 View