Will the magnetic beads (SIGMA-ALORICH; ANTI-FLAG M2 Magnetic Beads; Catalog Number:M8823) that suspended in 50% glycerol be freezed at -20 ℃? In my oponion, stored in glycerol is to avoid freeze, in order to decrease damage to the magnetic beads surface? Because the beads were borrowed from other lab, I have no idea about why it will be freezed? Does anyone who has ever used it? Is that okay to use? Really thanks a lot!
Sang Ho Lee
Hi, Riqiang.
If they came to frozen form, do not use!!! They lost surface activity of the beads. Glycerol prevents freezing and proteins conjugated to the surfaces in longer term storage at 4C.
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Amin Shavandi
They might be in a slurry and should not Freeze, but they have a shellfire which might expired or damaged.
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