Ricardo Peguero

3 Questions 4 Answers 0 Followers

Questions related from Ricardo Peguero

Will Evans Blue staining of a cell pellet interfere with downstream immunogold TEM?

I am doing TEM on a rare cell population (10k cells) - I am aware of methods for processing such small samples for TEM via agar embedding the pellet and using Evans blue to visualize the pellet...

22 December 2022 7,371 0 View

Can bad resin infiltration/polymerization lead to this appearance of sections in TEM? if not what could be the issue?

The attached images are ultrathin sections of a cell and whole embryo of a filarial worm stained with uranyless and lead citrate. I have fixed/processed/imaged similar samples dozens of times with...

07 October 2022 8,923 2 View

Can qPCR log2 calculations be done without normalization to a housekeeping gene?

I have done qPCR with 3 groups: 1 control, and 2 drug conditions, this qPCR only targeted one gene without being done with a reference gene. My questions is: If I detect a change in the log 2...

06 October 2022 3,758 9 View