Dear all,
I need to dissolve templates for further analysis, namely for Sanger sequencing. It is acceptable to dissolve templates in DEPC-treated water?
PS should I use Tris buffer (10mM Tris HCL, pH=8, 1mM EDTA) instead of DEPC water?
thanks in advance,
Piotr
DEPC treated water isn't suitable for sanger sequencing and dilution for primer when you use it for sanger. We use DEPC treated water only for rt-pcr. You could dissolve your templates in ULTRA pure water, which is RNase and DNase free.
Cheers.
I am currently out of 'nuclease free' water, and wondering what kind of diluent I should use for my templates.
I have lots of DEPC-treated water, so this is the reason I am asking question considering possible influence of DEPC on Sanger sequencing process.
In this case it would be reasonable to use tris buffer as a diluent.
You could buy double distiled water from pharmacy for diluting your primers.
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