I'm using an isolated heart model by perfusing the heart with oxygenated Hepes buffered Tyrode's Solution (the heart is contracting and looks healthy), and then I use vibratome to cut 350-500 um slices of rat heart (cutting in ice cold HBSS).
Then i transfer them to a warm oxygenated Hepes buffered Tyrode's Solution.
Should the slices contract spontaneously? or do i need to provide electrical stimulation?
Please, study the excellent protocol of work with myocardial slices (in Attachment).
Thanks you very much Vladimir, This is a very useful protocol.
However, i couldn't find a direct answer to my question.
They only mentioned that if cultured for long duration they will lose their contractility since they aren't stimulated. But i was wondering whether it is the case also for acute experiments, where the slices are imaged for 1-2 hours.
Do you know if they will contract spontaneously?
Best,
Hemi
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