Hemi Rotenberg

5 Questions 5 Answers 0 Followers

Questions related from Hemi Rotenberg

Sutter's Integrated Patch-Clamp Amplifier Vs. AM-system, any recomndations?

Hello, I'm building a new patch-clamp rig and wanted to know if anyone can compare the two of these. I'm interested in the user interface, stability, and overall experience. Many thanks Hemi

17 November 2021 2,268 1 View

Is there a dopant redistribution during RIE etching?

I'm using heavily doped p-type silicon (boron), and i was wondering if there is a dopant redistribution at the surface of the etched substrate. I was able to find this for wet thermal growth of...

23 August 2019 8,166 5 View

Why can't I get calcium live imaging from my cardiac slices?

Hi all, I'm using a vibratome to cut heart slices, and I want to do calcium imaging of the slices. I know for sure that the tissue is alive, as it is obviously contacting upon stimulation (without...

12 October 2018 2,710 3 View

Will cardiac slices contract spontaneously?

I'm using an isolated heart model by perfusing the heart with oxygenated Hepes buffered Tyrode's Solution (the heart is contracting and looks healthy), and then I use vibratome to cut 350-500 um...

22 August 2018 6,070 2 View

How to prform Immunostaining for Connexin-43 (rat cardiomyocytes) for flow cytometry?

I'm trying to see expression of Cn-43 in rat cardiomyocytes, using flow cytometry. i was also hoping to see expression in the cardiac fibroblasts that are also in the co-culture. Did anyone try...

19 February 2018 5,368 3 View