Presence of protein in a cell line that is used as a negative control for that specific protein?

I have quite an absurd issue. for the information: 1) I have a cell line (K), that i have seen in literature being used as a negative control for my protein (P). However, for me the cell line...

30 May 2019 3,213 3 View

Different genotyping result for the same sample under same conditions?

27 February 2019 331 13 View

Total RNA stability at room temperature (+25 C)?

Hello everyone, I'm facing a problem regarding my pure extracted RNA samples, I extracted them from tissues of rats' livers and kidneys immersed in RNAlater, using the promega SV isolation...

04 November 2017 3,737 5 View

Quality costs in different phases of slip casting?

05 October 2016 5,317 3 View

Comparing different qPCR amplicons, how to account for different length of amplicons when using SyberGreen?

Dear all, I performed several qPCR, which also worked fine, however, now during the evaluation I encountered an issue. My problem is that due to the restriction when choosing primers, I ended up...

05 August 2014 3,406 2 View

For ELISA, which capture/coating AB concentration should I use?

I am developping an ELISA, which seems to work, although I am puzzled by the titration of the capture AB. The Assay: All samples were in dublicates, volume used 50ul, except 150 for blocking,...

08 April 2014 6,566 3 View

Is there any evidence that links bacteria with oral cancer?

Certain microbes have been linked with oral cancer like HPV and syphilis. Recent research suggests that anaerobic bacteria may be linked with colon cancer.

26 August 2013 698 24 View

I focus for a training course

I'm a PhD student and I'm working on "Invasive Alien Plants in Tunisia: invasiveness and management strategies." I hope to have the opportunity to benefit from a training period in a laboratory...

17 December 2012 8,704 2 View

Can we modify the pre designed Deep networks like googlenet and alexnet to use them for signal classification?

Signal classification using Deep Learning

01 January 1970 9,370 4 View

What affects will the internet of things (IoT) have on infrastructure and smart cities region?

Is the competencies of the clever grid, smart homes, and its combined with IoT components in other public utilities, including roadways, sewage, and water transport can make a contribution to...

01 January 1970 7,751 5 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How to sample data to increase presence of solar panels in the images?

I am working on creating deep neural network on geospatial dataset and wanted to know how to do the sampling so that I can increase the presence of solar panels in the images. I cannot use entire...

25 February 2021 3,936 6 View

Satellite orbit estimation using Recurrent Neural Network - what should I use?

Hello, I am a master's student studying in Yonsei Univeristy, Korea. I am trying to estimate the state of satellite, using Neural Network. Below is a simple flow of my study. 1. Train (t0 ~ t1)...

23 February 2021 6,383 3 View

Discuss the extent to which morality in African traditional societies can be classified under the Natural Law theory of morality?

How can one classified African traditional societies under natural law

18 February 2021 7,334 8 View

Any methods to train a neural network with random (stochastic) and non-measurable variable?

Dear scholars, I was wondering if there are any scientific methods to train a neural network or ANFIS when one of the input variables is stochastic (random) and more importantly non-measurable?...

17 February 2021 1,909 3 View

Does anyone know what are the pros and cons for using autoencoders instead of CNNs for features extraction in neural networks?

Are there papers that talk about the possible advantages and disadvantages of using autoencoders instead of CNNs for feature extraction in neural network?

15 February 2021 6,952 3 View

What are the popular techniques for pre-processing measurement data in Smart Grid/Power System?

The data collected from various measurement devices often contains erroneous values - such as noise, missing values, etc. What are the popular techniques for pre-processing measurement data in...

14 February 2021 4,642 2 View