There is a problem in my research. When I mixed tannic acid with proteins in ddH2O, the complexation would form stable nanoparticles. After that, I added PLL (a kind of polycations) into the system, it seems good. But obvious aggregates formed after dilution with PBS buffer.
Besides, I found that the mixture of PLL and Tannic acid without proteins was good in ddH2O. However, the mixture in PBS buffer would aggregate and the solution turned cloudy.
I am wondering why the system is unstable in PBS buffer?
I am looking forward to your reply!
TO Vinay S P : Thanks for your reply! By the way, could you please tell me the detailed reasons?
I have no experience with the particular nanoparticles. Nonetheless if you see aggregation after adding PBS then the nanoparticle are not stable enough. Is this the reason you added the charged polymer? To impart stability?
To Omar Gonzalez-Ortega : I really appreciate your reply! I will think about the point you mentioned carefully. Thanks!
maybe that precipitation could be caused by electrostatic shielding due to dissolved salts of buffer. Should then be reversible after dilution with deionized water.
TO Titus Sobisch : Thanks for your help indeed. I fully agree with your thoughts. This should be the true reason. However, could you give me a suggestion about how to help stabilize the polycation complex in PBS buffer? I am looking forward to your reply!
maybe instead of using the buffer adjust pH by dilute acid / base?
Otherwise you would need steric stabilization, e.g. by adding polymer like PEO - no idea, which one would work
TO Titus Sobisch :Thanks indeed! I will have a try on it. Thanks again!
- Badges
- Science topic
- Similar topics
- Chemistry
- Nanotechnology
- Nanostructured Materials
- Nanoparticles