Why we need mapping population for marker assisted selection instead of normal population ?

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Anybody here who knows tool for custom designing of probe(target enrichment)?

Hi i want to capture 31Mb region in wheat for next generation sequencing. I asked for companies like Agilent and Nimblegen, but they are very costly. Therefore, does anybody know any tool like...

08 September 2019 6,520 0 View

How much primer designing differs from probe designing for exome sequencing? What if we design biotynylated primer instead of probe for capturing?

What are the criteria for designing biotinylated probes differ from primer designing?

08 September 2019 9,873 2 View

What to do next if your large no. of plant sample showing degraded DNA with long smear band ?

I have isolated DNA from 382 plants without running them on gel . I thought i will run them together after one month of completed extraction i found a long band of smear on my gel for all of...

02 March 2019 8,019 10 View

How to isolate large scale DNA samles smartly in a less time for SSR marker identification ?

hi, i am isolating DNA from 384 lines in a mapping population , but it takes my entire day for just 15 or maximum 30 samples . I want to do more than 30 in one time . Currently i am using 2...

01 February 2019 607 5 View

What experiments one can do for comparative analysis of adaptive mechanism in plant, along different altitude levels in harsh environment conditions ?

we are try to comparing total cellular content like chlrophyll ,carotenoid ,anthocyanin, proline ,sugar ,antioxidant enzymes of medicinal plant at different altitude level of Himalayas ,...

06 July 2018 6,007 8 View

Which subject listed below of plant science have maximum contributions in crop improvement?

In between Genetic and plant breeding, plant physiology, agriculture biotechnology and plant pathology which one subject have maximum contribution in crop improvement? Which one is better for...

03 April 2018 6,110 45 View

Can we feed the world in 2050?

As we all know world population will rise from 7.5 billion to 9.5 billion in 2050 and that will increase the total global food demand from 50 % to 97% according to FAO . What is your perspective...

01 February 2018 8,415 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Prahalada GD

Hello,

Mapping population like F2 possess variants (recombinants) which is crucial for associating phenotype and genotype.

Thanks

Rudra Bhattarai

could you please, expain clear about normal pop you mean for? Normally, QTL Mapping need two different groups such as resistance and suceptible which is only possible by using the mapping pop.