Shubham Bhardwaj

8 Questions 8 Answers 0 Followers

Questions related from Shubham Bhardwaj

Anybody here who knows tool for custom designing of probe(target enrichment)?

Hi i want to capture 31Mb region in wheat for next generation sequencing. I asked for companies like Agilent and Nimblegen, but they are very costly. Therefore, does anybody know any tool like...

09 September 2019 6,639 0 View

How much primer designing differs from probe designing for exome sequencing? What if we design biotynylated primer instead of probe for capturing?

What are the criteria for designing biotinylated probes differ from primer designing?

09 September 2019 9,995 2 View

What to do next if your large no. of plant sample showing degraded DNA with long smear band ?

I have isolated DNA from 382 plants without running them on gel . I thought i will run them together after one month of completed extraction i found a long band of smear on my gel for all of...

03 March 2019 8,143 10 View

How to isolate large scale DNA samles smartly in a less time for SSR marker identification ?

hi, i am isolating DNA from 384 lines in a mapping population , but it takes my entire day for just 15 or maximum 30 samples . I want to do more than 30 in one time . Currently i am using 2...

02 February 2019 713 5 View

What experiments one can do for comparative analysis of adaptive mechanism in plant, along different altitude levels in harsh environment conditions ?

we are try to comparing total cellular content like chlrophyll ,carotenoid ,anthocyanin, proline ,sugar ,antioxidant enzymes of medicinal plant at different altitude level of Himalayas ,...

07 July 2018 6,150 8 View

Which subject listed below of plant science have maximum contributions in crop improvement?

In between Genetic and plant breeding, plant physiology, agriculture biotechnology and plant pathology which one subject have maximum contribution in crop improvement? Which one is better for...

04 April 2018 6,271 45 View

Why we need mapping population for marker assisted selection instead of normal population ?

Plant breeder use F2 ,F2:F3, NILs,RILs , DH in MAS but how these population are most suitable for it.

03 March 2018 8,106 2 View

Can we feed the world in 2050?

As we all know world population will rise from 7.5 billion to 9.5 billion in 2050 and that will increase the total global food demand from 50 % to 97% according to FAO . What is your perspective...

02 February 2018 8,527 3 View