Does anyone have matlab code how to use k-means clustering using feature extraction as an input, please ? and also how can we get the c1, c2, ..,cN?

hi, i'm really new to matlab and k-means clustering. i'm sorry for my question but i really get lost now. if anyone can help me or have matlab code how to use k-mans clustering using feature...

09 October 2018 2,954 8 View

How cFDA stain Plasmodium-infected RBCs?

Hi, We performed an experiment to measure phagocytosis assay with cFDA to stain the Plasmodium-infected RBCs. I just wonder, is cFDA stain the parasite inside RBCs or only stain the RBCs? Thank you

18 March 2018 7,636 3 View

Is there a role for cultural activism in challenging orthodox research methodologies ?

Black Emancipatory Action Research includes at its core participatory and action research methodology which imperatively rely on community participation and community empowerment at all stages of...

23 June 2017 2,775 8 View

How to interpret the CV curve of cathode for Li-ion battery?

16 June 2016 5,003 3 View

Has anyone analyzed the severity of duty cycle in accerlated life testing for automoblie durability qualification?

11 November 2015 2,485 3 View

Can anyone give me some informations about the cyclic voltammetry of LiPF6 electrolyte?

Dear colleagues, I'm currently doing a research about Li-ion battery. In this case, I used LiFePO4 as a cathode. I got a peak of reduction around 0.8 V. I supposed that the cathodic peak seen...

12 May 2015 2,686 1 View

How can I synthesize ZSM-5 Free Structure Directing Agent by using hydrothermal technique?

I have synthesized some compounds by different variations of Si/Al, OH/Si, H2O/Si, temperature, condition (tumbling and non-tumbling) but I got almost the type of mordenite. There is also ZSM-5...

03 March 2015 6,411 6 View

Can anyone explain how to read the diagram of cyclic voltammogram?

I have diagram of cyclic voltammogram, seeking to understand how to define the diagram voltammogram.

06 May 2014 8,990 10 View

how can the voltage range be determined in aqueous electrolyte (Li2SO4) with LFP cathode and LTP anode materials?

Aquaeous electrolyte for lithium ion batteries

31 March 2014 5,218 1 View

How can I calculate mass of carbon in LiFePo4/C?

.

15 February 2014 4,584 7 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View