Why total RNA shows one strong band on the gel?

03 November 2021 1 9K Report

I extracted total RNA from some acetone fixed tissue using miRVana miRNA isolation kits. When I ran it on the regular TAE gel (1.5%) it showed only one clear band (~800bp) but no expected bands corresponds to the rRNAs? Is my sample degraded?

Shen Tian

I just leant that its probably because I heated the sample at 95 for 3min before loading onto the gel. I’m working on an insect species, and it was reported in multiple studies that in some insects 28s would be cleaved by higher temperature and generates two fragments of the same size of 18s…

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