Hello,
I am facing with a problem and it occurs many times. I am tring to prepare a terrific broth from normal recipe, using buffer salts, yeast extract, tryptone and glycerol at the end. Although I use distilled water and prepare my medium freshly, even after autoclave my medium seem to be cloudy and when I wait, there is white precipitate at the bottom. I changed the solid ingredients thinking that they may absorbed some moist from air, I couldn't solve the problem.
Does anyone have any suggestion/reasoning for this problem ?
Thank you in advance!
That might be from the phosphate in your buffer. Have you tried to filter sterile phosphate buffer and add it to the media just before using? In addition, the precipitation could derive from the interaction between magnesium and the phosphate buffer.
I hope this helps.
I did not try filtering before. I will try this way then. Hope it helps.
Thank you
Hello Jenny, it reduced but didn't diminished actually. The quality of salts and ingredients however is important. If they are like moistured or if anything hygroscopic stored under air etc it affects. If you face with same thing I recommend you to check the qualities of them and filter water before adding to the broth. Good luck!
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