There is very little publication where functional characterization(cloning, overexpression, silencing, etc.) of genes identified through GWAS has been performed. However, most of the publications on functional characterization are on genes identified through transcriptome. Why is this? I doubt whether there is any usefulness of GWAS on crop improvement or not? if yes then give me some successful publication examples?
Interesting question
may be this is because most publications on GWAS analysis are dated 2015-2021, especially on tree crops. Maybe this is too short period to successfully validate the revealled candidate genes. Possibly many articles on validation topic will be released in near future...
The reason is that there are very few publications where functional characterization (cloning, overexpression, silencing, etc.) of the genes identified through GWAS has been performed. However, most of the publications on functional characterization revolve around genes identified through transcription because these are quantitative traits and are controlled by many genes and the influence of the environment is very high and the effect of each gene is weak (Minor genes) and they have small-effect genes rather than Major genes that have large -effects