Shubham Bhardwaj

6 Questions 7 Answers 0 Followers

Questions related from Shubham Bhardwaj

Why there is not much publication about cloning and functional characterization of genes identified through GWAS in plant ?

There is very little publication where functional characterization(cloning, overexpression, silencing, etc.) of genes identified through GWAS has been performed. However, most of the publications...

18 October 2021 1,550 3 View

How much primer designing differs from probe designing for exome sequencing? What if we design biotynylated primer instead of probe for capturing?

What are the criteria for designing biotinylated probes differ from primer designing?

25 September 2019 8,324 2 View

What to do next if your large no. of plant sample showing degraded DNA with long smear band ?

I have isolated DNA from 382 plants without running them on gel . I thought i will run them together after one month of completed extraction i found a long band of smear on my gel for all of...

14 March 2019 3,567 9 View

How to separate xanthophyll from other carotenoioid mixed in petroleum ether ?

In 1st step we have extracted plant pigments in 100 %acetone . Step 2 ... Acetone extract is mixed with petroleum ether in separotory funnel . Step 3 ......Acetone containing chlorophyll is...

03 August 2018 6,456 3 View

Which subject listed below of plant science have maximum contributions in crop improvement?

In between Genetic and plant breeding, plant physiology, agriculture biotechnology and plant pathology which one subject have maximum contribution in crop improvement? Which one is better for...

22 April 2018 9,733 47 View

Why recombination frequency is not more than 50%? Does that mean two genes cannot be more than 50cM apart?

i am not able to find easy explanation of this question . Some people says , this is because only one sister chromatid of homologous chromosomes cross over with each other.

01 January 1970 4,617 3 View