Shubham Bhardwaj

10 Questions 9 Answers 0 Followers

Questions related from Shubham Bhardwaj

Why there is not much publication about cloning and functional characterization of genes identified through GWAS in plant ?

There is very little publication where functional characterization(cloning, overexpression, silencing, etc.) of genes identified through GWAS has been performed. However, most of the publications...

18 October 2021 1,560 3 View

Why some transcription factors have joint name for e.g. AP2\ERF, AREB\ABF and DREB\CBF etc?

Why these transcription factors have fused name from two different protein For e.g AP2 play their role in flower development and ERF is ethylene response factor, But in ABA signalling in abiotic...

06 April 2021 2,911 1 View

How many genes in human genome have known identified function?

We all know that human genome have 20000 plus genes. That's we know through structural annotations. But I like to have an estimate how many gene among them have clear known assigned function....

27 December 2020 8,042 3 View

How much primer designing differs from probe designing for exome sequencing? What if we design biotynylated primer instead of probe for capturing?

What are the criteria for designing biotinylated probes differ from primer designing?

25 September 2019 8,351 2 View

What to do next if your large no. of plant sample showing degraded DNA with long smear band ?

I have isolated DNA from 382 plants without running them on gel . I thought i will run them together after one month of completed extraction i found a long band of smear on my gel for all of...

14 March 2019 3,595 9 View

How to separate xanthophyll from other carotenoioid mixed in petroleum ether ?

In 1st step we have extracted plant pigments in 100 %acetone . Step 2 ... Acetone extract is mixed with petroleum ether in separotory funnel . Step 3 ......Acetone containing chlorophyll is...

03 August 2018 6,524 3 View

How to interpret mass spectrum of MALDI-TOF manually without software ?

How can we determine protein sequence from mass spectrum manually ?

08 July 2018 3,903 2 View

Which subject listed below of plant science have maximum contributions in crop improvement?

In between Genetic and plant breeding, plant physiology, agriculture biotechnology and plant pathology which one subject have maximum contribution in crop improvement? Which one is better for...

22 April 2018 9,753 47 View

How much plant scientists are successful to reduce the gap between plants potential yield and its actual yield due abiotic stresses?

Scientists are still not getting very much successful in designing stress tolerant crops even after applying conventional ,omics and transgenics approaches to stress tolerance . .What are the...

23 January 2018 9,722 4 View

Why recombination frequency is not more than 50%? Does that mean two genes cannot be more than 50cM apart?

i am not able to find easy explanation of this question . Some people says , this is because only one sister chromatid of homologous chromosomes cross over with each other.

01 January 1970 4,632 3 View