Puspita Saha

5 Questions 5 Answers 0 Followers

Questions related from Puspita Saha

Why the same viral protein is getting expressed in the A549 cells but not in the HEK293T cells when I am using the same expression vector pcDNA3.1?

Does pcDNA3.1 vector not work for HEK293T cells?

18 March 2022 3,650 2 View

How is it possible that N-terminus of the protein is getting degraded while C-terminus is getting expressed with the fusion tag?

I am using pcDNA3.1 vector for overexpressing viral protein in mammalian cells.

10 March 2022 9,611 4 View

I am not getting the overexpression band of viral protein in mammalian cells on performing transient overexpression. What should I do?

Please suggest some way other than codon optimization.

10 March 2022 4,358 0 View

What is the rationale behind intramuscular administration of vaccines?

As we all know there are different routes of vaccine administration, I am specifically interested in knowing what kind of specific immune responses are triggered by intramuscular (im) injection of...

24 July 2020 2,650 0 View

At which genomic locus of host genome does the multicistronic 2A peptide based retroviral vector get integrated?

I want to understand the designing strategy for integration of the retroviral vectors used for generation of retrogenic mice. What are the sequences that facilitate their integration in the host...

29 June 2019 2,556 1 View