Why the intramolecular hydrogen bonds showed this much of difference between Apo protein and protein complex?

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I am trying to resolve immune cytokines. Which is the right percentage of SDS resolving gel?

I wish to resolve IL-6 (mol wt= 21-26KDa), TNF- alpha (17.3-17.4 KDa), GMF-beta (16.7 KDa), GAPDH (36kDa) and PDL1 (40-70 KDa). I think 12.5% gel is good to go. Is it advisable or should I go for...

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How do i calculate cut off values in ELISA?

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Need to justify choice of two deep learning models CNN and sae compared to other models in data analysis ?

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Need to justify choice of two deep learning models CNN and sae compared to other models in data analysis ?

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18 January 2024 9,816 0 View

Which primers should I use for random amplification of bacteria from CSF sample?

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(How) Is it feasible to use a questionnaire (of closed ended questions) to explore the lived experiences of participants in a phenomenological study?

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Can anyone suggests a free software for finding particle size ?

For the measurement of particle size from XRD TEM SEM etc

12 November 2023 8,821 2 View

Is there any alternate way to avoid perfusion using a perfusion buffer for isolation of liver cells for primary culture?

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Can anybody provide me a reference about, TAHARA task which is based on the flow solver created by Prof. Yusuke Tahara of Osaka Prefecture University?

Required to understand how to solve potential flow around a ship hull.

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What percentage of scientific papers get more than 200 citations?

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10 September 2023 5,973 1 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

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Why does my protein refolded to beta sheet during thermal denaturation analysis?

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Why results of ROS flurescence are negative as there was no bacteria within?

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How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

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What is the ionic radius of Hydrogen anion? And how to know the exact ionic radii of the elements?

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Transfection in HEK293T cells?

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31 July 2024 9,892 4 View

How to make the concentration measurements for the hydrogen absorption/desorption in the metal hydride?

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29 July 2024 7,577 2 View

How to retain the a GFP tagged gene expression in stable cell line?

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What is the relationship between protein structure and N or C terminal tagging choosing?

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28 July 2024 366 3 View

What change would occur in physics if the three different sizes of the proton and the two sizes of the deuteron accepted as new physical constants?

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28 July 2024 7,823 1 View

Jürgen Weippert

How was this created? Is this a molecular dynamics result? Can you look at the snapshots over time?

Soundar Rajan Kulandhaivel

Hai Jürgen Weippert , this image was generated from gromacs. Simulations were performed for apo and complex of same protein. But the intra-molecular hydrogen bonds plot showed much deviation. I dont know the reason for this deviation. I run the simulation for another one protein, the result of that protein is fine.

I am trying to resolve immune cytokines. Which is the right percentage of SDS resolving gel?

How do i calculate cut off values in ELISA?

Need to justify choice of two deep learning models CNN and sae compared to other models in data analysis ?

Need to justify choice of two deep learning models CNN and sae compared to other models in data analysis ?

Which primers should I use for random amplification of bacteria from CSF sample?

(How) Is it feasible to use a questionnaire (of closed ended questions) to explore the lived experiences of participants in a phenomenological study?

Can anyone suggests a free software for finding particle size ?

Is there any alternate way to avoid perfusion using a perfusion buffer for isolation of liver cells for primary culture?

Can anybody provide me a reference about, TAHARA task which is based on the flow solver created by Prof. Yusuke Tahara of Osaka Prefecture University?

What percentage of scientific papers get more than 200 citations?

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Why results of ROS flurescence are negative as there was no bacteria within?

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument​?

What is the ionic radius of Hydrogen anion? And how to know the exact ionic radii of the elements?

Transfection in HEK293T cells?

How to make the concentration measurements for the hydrogen absorption/desorption in the metal hydride?

How to retain the a GFP tagged gene expression in stable cell line?

What is the relationship between protein structure and N or C terminal tagging choosing?

What change would occur in physics if the three different sizes of the proton and the two sizes of the deuteron accepted as new physical constants?

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?