Why the data that I am getting in my yeast spot dilution assay is not the same with liquid culture growth curve assay??

Madhuparna Chakraborty @Madhuparna-Chakraborty

29 November 2021 0 1K Report

I am checking toxicity of my desired protein in two different genetic backgrounds in yeast system by Spot dilution assay. The observation which I am getting is not similar to the data I am getting in liquid growth curve assay for 24-48 hours. What can be the possible explanation for this difference in results of two different methods measuring same parameter???

Badges
Science topic

More Madhuparna Chakraborty's questions See All

Does there exist any empirical evidence of government expenditure financed by money collected through lobbying?

Basically, i am trying to understand from the literature whether lobbying can also considered an alternative source of financing fiscal deficit.

26 July 2024 8,692 5 View

How to determine method detection limit in an analytical method?

I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...

24 July 2024 6,592 5 View

RNA isolation from plant roots infected with both bacteria and fungi. How to isolate RNA from all of them simultaneously?

I have a plant whose roots have been infected with both bacteria and fungi. What strategy should I follow to isolate RNA of all three, i.e. plant, bacteria and fungi at once? This RNA is to be...

09 June 2024 9,876 0 View

How can I find capacity of Li-Ion coin cell (CR2023) battery ?

if I want to run my battery cycling test in 1 C rate ,but then I need to know the capacity of my material used in the coin cell (i.e. if my capacity is 20mAh then I will put a constant current of...

05 May 2024 1,364 1 View

Are there any suggestions or insights you can provide to help address this convergence failure in my optimization process?

I'm currently experiencing convergence failure while attempting to optimize a dimeric liquid crystal molecule under the influence of an electric field. The optimization code I'm using is...

10 March 2024 4,737 0 View

Does the dipole moment of a molecule correlate with its hyperpolarizability and polarizability?

implying that a molecule with a lower dipole moment exhibits lower hyperpolarizability and polarizability compared to one with a higher dipole moment?

07 March 2024 572 2 View

What is the script for running protein cluster by using DBSCAN?

Im trying to run dbscan.py by using vmd (dcd and pdb) files but the script is showing error. Its not generating cluster it's only generating noise from the trajectory file. How to solve this issue...

03 March 2024 6,414 0 View

How can I effectively analyze my data using multinomial logistic regression in SPSS?

I am conducting a study that involves analyzing categorical outcome variables with multiple levels and want to employ multinomial logistic regression analysis in SPSS for my data. However, I'm...

29 February 2024 8,533 4 View

Why is my IS high in plasma when measured in GCMS?

Hello everyone, I am currently working on developing a method to quantify butyric acid in plasma on single quad-GCMS using electron impact mode. For this purpose, I am currently following this...

14 February 2024 3,919 0 View

What are the key considerations and steps involved in the simple centrifugation method for isolating lymphocytes?

What are the key considerations and steps involved in the simple centrifugation method for isolating lymphocytes withou using ready to use lymphocytes seperating medium?

29 January 2024 3,222 0 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

How can I measure arsenic concentration in a patient blood?

I intend to get an economical and accurate LAB TEST to measure arsenic concentration in blood sample.

23 July 2024 3,654 2 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View