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Questions related from Madhuparna Chakraborty
I have isolated yeast Mitochondria following a standard protocol. But while checking the purity of mitochondrial fraction by Western blotting, I am getting a histone 3 band in addition to a hsp60...
25 April 2023 2,626 3 View
I am doing MTT assay of TDP-43 transfected HEK293T cells in a 6 well plate. I am dissolving the MTT in DMEM media without Serum and Phenol red. After adding 850ul of the MTT solution to the cells...
05 April 2021 953 4 View
I am transiently transfecting HEK293T cells with pEGFPC1 WT TDP-43 construct using PEI transfection reagent. After 40hrs of transfection, I am staining the cells with annexin v apc and Propidium...
30 March 2021 5,008 5 View
Our lab is facing a difficult problem for almost a year. After thawing the cells are okay, but after 2nd or 3rd passage there are debris present between the cells. We use mycoplasma removed cells...
18 January 2021 5,827 6 View
I want to mutate serine 409 and 410 into aspartic acid. For that two bases have to be changed for converting one serine into aspartic acid. So in my case four bases will be changed and between two...
19 August 2020 3,476 4 View
I haven't changed anything in the protocol for cell culture..
21 May 2019 9,648 6 View