what different roles do Ca and Mg play in protein extraction and purifying buffers, ip wash buffers , etc.
for example, in the link, concentration of Mg and Ca changes in different wash buffer
Sebastian Pintscher
micrococcal nuclease needs Ca2+ ions to be active, that's why you need them in TGMC buffer from this protocol
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Tausif Alam
In cases where one is focussing on a specific enzyme that can use either Ca or Mg ions almost equally well, investigators may use and report the composition of a buffer that is commonly used their labs for other reasons. Similarly, if one can use Na+ instead of Li+, there is a cost difference, NaCl being much cheaper.
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