Hello,
I would like to ask your opinions about Alizarin red staining. I am new for the technique. In most of publications the photo of Alizarin red staining shows really red color, however, when I try the protocol, the color is not intensive enough, and is brownish even a bit yellow. Does anyone has idea about it?
I have used alizarin red 2% ph 4.3. The osteoblast has been cultivated with ascorbic acid and glycerol phosphate for 3 weeks, and fixed by 3.7%PFA or Methanol.
Best regards