Why Terrific Broth gets precipitated as soon as i add TB salts (0.17 M KH2PO4, 0.72 M K2HPO4) is added?

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Asst.Prof.Muthanna Hadi Hussain

You should keep the solution at 50C0...

Mayank Pokhriyal

i have cooled it below 50 degrees also ..still it gets precipitated after adding TB salts

Yung-Tsung Kao

You can autoclave the Yeast extract, tryptone and pH salt: K2HPO4, KH2PO4 separately. It will no precipitation anymore!

https://www.carlroth.com/medias/Info-Brochure-Phosphates-in-Media-EN.pdf?context=bWFzdGVyfHRlY2huaWNhbERvY3VtZW50c3wyNTc3MTl8YXBwbGljYXRpb24vcGRmfHRlY2huaWNhbERvY3VtZW50cy9oMzEvaDk3Lzg5NzY2Mjk5NTY2MzgucGRmfDZmMGRiOTE1YWUwYmFlZWM5M2QxZWI5ZTRkNjk2MzFmNGFjMmM0ODNiNDEzYzk5OGY0MDRkMjJiNjY5ZmEwM2Q