Why some genes first clone intp p-Twist vector through Gibson asssembly and then clone into p-BAD through Golden gate assembly method?
How to enter the extinction coefficient in the calculation of the amount of anthocyanin?
15 June 2024 1,181 1 View
Call for Editors: TESOL Today TESOL Today, a distinguished scholarly journal at the forefront of Teaching English to Speakers of Other Languages (TESOL) and Applied Linguistics, is seeking...
08 May 2024 1,070 3 View
Dear TESOL and Applied Linguistics researchers, Greetings, TESOL Today invites educators, researchers, and practitioners in the field of Teaching English to Speakers of Other Languages (TESOL)...
02 May 2024 244 3 View
Practically it forms gel
10 March 2024 3,127 1 View
As i am doing multipass simulation with APDL Subroutines and at that time i had no idea how can i apply Element birth and death technique for simulation. And what logic should i used for the...
23 February 2024 3,833 0 View
study about evolution of skull of viperidae. some body help me about this.What studies have been done in this regard so far?
20 February 2024 4,804 0 View
We are looking for a research group that contains a research group in the field of management and human resources?
16 February 2024 9,102 6 View
I am working on a meta-analysis that examine the dose-response association of fasting plasma glucose (a continuous variable) on incident cardiovascular diseases. Although in most of the original...
06 February 2024 9,277 1 View
Does anyone know the latest DEA approaches to evaluate Environmental Efficiency? or what do you propose for that?
17 December 2023 5,871 2 View
I'd like to open a discussion on the correlation between particle size and the performance of pyrolysis. What are your insights regarding the influence of particle size on the efficiency and...
20 November 2023 8,816 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
As flaviviruses genome is very frequently prone to mutation hence it is very difficult to clone whole genome inside vector what kind of vector and cells can sustain such amount of cloning...
11 July 2024 4,284 0 View
I am working on cloning of a gene fragment inside my vector everything we tried to do correctly like making insert and vector with utmost care but after ligation the morphology of colonies seems...
11 July 2024 9,010 3 View
This question seeks to address the growing concern of cloned academic journals, which are fraudulent duplicates of legitimate publications. It aims to guide researchers, scholars, and academics on...
10 July 2024 5,966 2 View