Selecting particular columns from large datasets (csv) using R Studio?

So I have large number of csv files (100+) in a directory with same structure and column names. The data is in time series and there are approx 30 columns/file. However i only want to aggregate...

08 February 2021 245 5 View

Why a methanolic semi solid plant extract when again dissolved in methanol does not give a homogeneous solution?

A clear methanolic extract solution obtained after filtration when concentrated gives a semi solid extract. Now this extract for a quantitative analysis needs to be taken by weight and made a...

30 December 2020 2,778 7 View

How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for Metabarcoding?

I'm working on pollen DNA metabarcoding to identify the floral composition of honey. How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for the...

28 December 2020 9,132 1 View

How to add two vertical and parallel Fresnel reflection coefficients to retrieve overall reflection coefficient for a right hand circular wave?

I have a problem in which a right hand circular polarized wave (GPS wave) encounters an ideal smooth plane obliquely, and then is reflected off that. To solve this kind of problems, the wave is...

28 December 2020 2,271 3 View

Which is better to focus on teaching or research for a good academic life and Why?

Which is better to focus on teaching or research for For a good academic life and Why?

26 December 2020 5,737 9 View

Is ag paste suitable for back contact on silicon wafer? or i should deposit al on the back surface using pvd or plasma sputtering?

Fabricating silicon solar cell

22 December 2020 4,906 7 View

Which renewable source is the best ?

wind hydro solar etc.

21 December 2020 6,360 16 View

Why is light pure energy?

light is pure because the light is the simplest electromagnetic construct but the energy of any object is also of the same kind.

20 December 2020 207 4 View

How to handle Missing Values for Skipped Questions (Systematic Missing) in R ?

10 December 2020 435 4 View

What is the best industrial emulsifier to make silicon oil in water emulsion?

I need to make long-lasting stable emulsions of 6 wt. % percent silicon oil in DI water. The previousely made emulsions using Tween and Span Emulsifiers did not remain stable after a week.

31 October 2020 3,568 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View

I am working on image transmission, i converted the image into binary vector, i want to extract the MSB of each pixel ?

i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?

19 February 2021 2,352 5 View

Why are Sanger Sequencing reads interrupted in some edited clones?

I have a Crispr/Cas9 edited bulk population and I can briefly check the indel ratios in the bulk population using Sanger sequencing. After cloning single cells, I wanted to check the indel types...

18 February 2021 8,198 6 View

Hi, can anybody help me with the suggestions how can I amplify a stress induced gene of 1107 bp?

I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...

17 February 2021 7,802 2 View