Can fractal dimension of thin film surfaces affects the electrons energy states?

As fractal dimension of a thin film surface implies the irregularity in the surface. Is it possible that the energy states of the electrons are also affected by the fractal dimension?

19 February 2021 5,509 3 View

Can anyone suggest papers in the hybrid auto-mata area that models cyber-physical systems in power systems?

Most of the hybrid automata papers target formal verification of the systems. I am looking for papers that are solving optimal response problems that propose optimal control in power systems under...

07 February 2021 5,233 1 View

Which lysis buffer should I use for HEK293T cells, which I have transfected for purification of a non-secretory (approx 200 kDa) protein?

Hi Everyone, I am a beginner at eukaryotic(HEK293T) protein expression. I am trying to purify a HIS tagged 200 kDa viral polyprotein using pCDNA3.1 vector. I am not sure which lysis buffer should...

11 January 2021 1,878 3 View

Anyone interested to help me building complete Civil Engineering Website??

30 August 2020 4,467 25 View

How does the pH of the solution affects the dispersion behavior of large sized graphene oxide nanosheets in various solvents?

I am trying to understand the dispersion behavior of graphene oxide in a range of organic and inorganic solvents to understand their specific applicability. Thus the pH dependent solubility...

26 August 2020 4,907 4 View

How to calculate average roughness, interface width and height-height correlation function using the XYZ data of AFM image in Matlab?

I have some XYZ data of AFM images for a thin film. I want to calculate the average roughness, interface width and height-height correlation function. How to do it in Matlab?

12 July 2020 6,248 1 View

What is the best graph based database for storing complex graphs from diverse domain?

This question is related to making inferences from a complex graphical model. Graph databases are used to store and query the relationship between nodes in a graph. We need an open-source graph DB...

18 June 2020 5,903 3 View

Has anyone faced a calibration issue of the electrobalance linked with KSV NIMA Langmuir Instrument?

Hi, I have been using the KSV Langmuir Trough and LayerBuilder for a long time now. Recently, we had to move the instrument due to renovations and I thought of calibrating it once again. We...

02 February 2020 7,946 1 View

Is there any correlation between term "very slightly soluble" as per USP and "poorly soluble" drug as per BCS Class II ?

Saturation Solubility (Equilibrium solubility) of drug (if between 0.1 to 1 mg/ml) in distilled water can this information be used as the predictor of the BCS Class ? How you determine BCS...

26 December 2018 6,742 5 View

Which is the most efficient glass surface cleaning protocol?

I want to clean the glass surfaces for surface modification analysis, therefore i am exploring various glass cleaning protocols for the maximum possible decontamination. ( Organic, inorganic and...

29 August 2018 4,982 4 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

Alternative to mediation analysis?

I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....

21 February 2021 8,609 1 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View