Why should I freeze a gel slice before RNA extraction?

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Microglia isolation of adult mice?

Dear all, i have been trying to isolate microglia of adult murine brain . i used a percoll density gradient; unfortunately it worked only in the first time for me, very well. after that.. it...

11 August 2021 5,508 5 View

Same variable, indirect effect is negative, moderating effect is positive. What does this mean?

Mediator is negative, but moderator is positive, how? These were the results of the mediator analysis: X - M is significant positive M - Y is significant negative My indirect effect is negative,...

15 June 2021 1,067 4 View

How to calculation energy expenditure from different movement behaviors in children?

I am hoping to estimate energy expenditure (kJ or calories) from actigraph reported movement behaviors (i.e. LPA, MVPA, SB) in minutes, for children age 8-12 years. The data was collected with the...

01 June 2021 2,545 0 View

Does anyone know where to obtain Acrolein? (2-Propenal)?

Please, i need urgent help. do you know where to buy Acrolein? Sigma used to sell this, but it is not available anymore. i checked elsewhere, but without success. please help! thank you silke

22 September 2020 3,291 8 View

GFP positive cellline without any transfection/ modification?

Helloo, maybe someone here can help me. i have acellline which gives me at baseline a clear FITC signal, i did not perform any transfection/modification to this line. can you explain this?

14 April 2020 3,705 0 View

Potassium levels in pediatry?

hello, i need some help. i am searching for good reference values for pediatric laboratories for the potassium levels. in the newest version of "Thomas" i found a good table, but they did not...

05 February 2020 3,583 6 View

Hey! PLease help me out: do yoou know what happens if you add more Sodium azide in your primary antibody dilution? f.ex. 0,1%? any experience?

will the antibody binding be affected? (recommended is 0,02-0,05%) i would be happy to get some help

30 January 2020 7,790 1 View

Anyone knows someone interested in a PhD at the TUM in my Chronobiology/Immunology/Microbiota/Gut -group?

Thankful for any help!

24 April 2018 3,425 8 View

Leadership feedback scale - any suggestions?

I'm looking for a well-established scale to assess leadership competences by asking employees? any suggestions?? The best would be a validated scale Otherwise it would help to get dimensions that...

27 April 2017 6,493 4 View

Is there a validated Dutch/Flemish version of the Physical Self-Description Questionnaire (PSDQ) (Marsh, 1994)?

If not, how can I access the English version? I would like to use it for my Master's Thesis. Thank you!

18 October 2016 1,403 1 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View