31 January 2020 1 8K Report

will the antibody binding be affected? (recommended is 0,02-0,05%)

i would be happy to get some help

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Is there any free reliable software to simulate chemical interactions between polymers and other molecules?

Hello! I need to simulate and investigate possible chemical interactions between some polymers and dye molecules. Is there any free reliable software you would recommend? Thank you in advance....

29 January 2021 541 2 View

Quantitative research - hypothesis formulation necessary?

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Potassium levels in pediatry?

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How can you do statistics with normalized data?

Just briefly: I have WB quantifications from 4 different blots (4 biological repilcates) and I normalized my signal intensity values to the untreated ctrl sample. Therefore, for all 4 replicates...

14 July 2019 3,258 3 View

Internal normalization in Western Blot quantification and statistics. How to?

This might seem like a rather simple problem, but I am actually unable to find a helpful solution that specifically refers to this problem. I did several Western Blots of biological replicates...

10 July 2019 6,476 5 View

Is AmberliteXAD2 an suitable alternative for Bio-Beads?

We are currently looking for cheaper alternatives for removing detergent from proteins during reconstitution into membranes. Bio-Beads work nicely for us, but they are costly. We found now...

15 May 2018 559 2 View

Anyone knows someone interested in a PhD at the TUM in my Chronobiology/Immunology/Microbiota/Gut -group?

Thankful for any help!

24 April 2018 3,291 8 View

Can I selectively remove host cells from mouse blood while retaining intact bacteria?

To enumerate the total amount of bacteria per blood volume, I would like to selectively remove host cells from mouse blood while retaining bacteria. Size filtration is unlikely to work as filters...

22 October 2017 6,278 3 View

Preservation of bacterial transcriptome upon cooling?

Is there any study around on how well bacterial transcriptomes are preserved when they are taken out of the "in vivo" situation and kept on ice for varying amounts of time? In other words, how...

12 September 2017 2,450 2 View

Is there a validated Dutch/Flemish version of the Physical Self-Description Questionnaire (PSDQ) (Marsh, 1994)?

If not, how can I access the English version? I would like to use it for my Master's Thesis. Thank you!

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