My problem: sequencing result is low-quaility and dirty. Most sequence is matched with expected one, but there are some sites having two bases' signal, and the overall base signal is not strong, but around 60th bp position, there is a very high peak.
Please help me! I can give more information if needed.
Paul Rutland
Can you attach an original .abi sequence file. A big multibase peak near the start of the sequence shows poor post sequence reaction clean up and a weak signal may be an old sequencing kit or too little template dna or too little sequencing primer. These lead to a weak signal and this gets messed up with random background noise from the sequencer but these things are often easier to troubleshoot with access to an original sequence file
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