what are the binding forces that act between the antigen or antibody and the polystyrene plates that help in their binding?
Hello Nisha Reghu
Polystyrene microplates are a type of matrix commonly used for immobilization of proteins. Proteins passively adsorb to the polystyrene surface through hydrophobic interactions. Generally, this adsorption of proteins onto the polystyrene surface occurs best when one uses carbonate/bicarbonate buffer at an alkaline pH (9.0–9.5).
Polystyrene will bind a wide variety of proteins depending on their concentration in the coating buffer. Carbohydrates and those proteins that are heavily glycosylated do not adsorb well to polystyrene because they have very little ability to participate in hydrophobic interactions.
There are plates available that are either polystyrene or derivatives of polystyrene obtained by chemical modification or irradiation of the surface. The capture protein can be either passively adsorbed on the surface of the polystyrene plate or covalently coupled through modification.
Best.
Polystyrene plates are used for ELISA (enzyme-linked immunosorbent assay) because they are non-reactive, transparent, accurately dimensioned, and have a uniform surface. Polystyrene is also an economical choice for many ELISA applications, making it an ideal choice for research and development. The uniform surface allows for easy coating of the plate with antibodies or antigens, and the non-reactive properties make it an ideal choice for many biological assays.
There is not one specific but few reasons to use Polystyrene plates. As they provide a good surface for the antibodies or antigens to be immobilized. Also this material does not interfere while taking absorbance, hence provide better results.
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology
- Antibodies