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Questions related from Nisha Reghu
We are running western blot for checking the ag-ab interactions.our proteins are from molecular weight 250 to 7 kda.But we are facing some technical issues with the transfer of proteins(i'm not...
29 November 2023 1,385 2 View
I did agarose gel electrophoresis with different concentrations of Dna:250ng,500ng and 1 microgram.But the bands were in smear form,from which I concluded that the dna was degraded.But when i...
11 August 2023 6,924 4 View
what are the binding forces that act between the antigen or antibody and the polystyrene plates that help in their binding?
02 May 2023 6,247 3 View
how can we detect the presence of a g coupled protein receptor getting activated when there is a signal molecule received. how can we prove it experimentally apart from measuring the level of...
02 March 2023 8,557 1 View
Everytime I add the resolving gel, it gets leaked.so is there any remedy to prevent the leakage of the resolving gel?
23 December 2022 7,515 3 View
what is the principle behind the protease assay?
08 December 2022 9,409 5 View