How to Detect Water in THF (Tetrahydrofuran) ?

Hello, we have solvent purifier system in our lab. But There is some unwanted results for our experiment. We are suspecting purity of solvents. Are there any simple methods to detect water in THF?

19 November 2020 114 5 View

What is the best way to combine Landscape Architecture and Entomology?

I am trying to find a topic for my PhD thesis. I've done two MSc; one in Landscape Architecture (about Ecological Landscape Design and Permaculture) and one in Forest Entomology (about...

21 April 2020 5,286 2 View

If an animal feeds mainly on algae but also eats cyanobacteria and insects, which group does this animal belong to; herbivore or omnivore?

I wonder if a fish (Random sp.) feeds mostly on plankton but also consumes cyanobacteria and some insects (Chironomidae, Simuliidae) at much lower rates, can we classify them as omnivore?

03 March 2020 8,177 4 View

How does the amount of amino acid exceed the amount of crude protein in fish meal (Dry matter basis)?

I read a SCI article where the author analysed several fish meals' amino acid profiles and crude protein levels. I.E., The findings indicate that the crude protein level is 79.50g/100g for fish...

16 July 2019 2,039 6 View

Which one should I choose from the results of a sample with different amino acid analysis methods?

I have a protein source with varying results of amino acid profile. For instance; when the sample is hydrolysed with "6 M HCI" L-Valine level is 0.24g/100g whereas it is 3.08g/100g when...

23 February 2018 5,648 2 View

What is the difference between higher order (second order) confirmatory factor analysis and bifactor analysis?

24 July 2017 5,178 5 View

How to determine total RNA concentration when you have large amount of miRNA?

I am isolating total RNA with miRNeasy micro (PBMCs) miRNeasy serum plasma (serum) and exoRNeasy (serum/exosome) for Next Gen Sequencing for miRNA. I believe my final product should contain mostly...

30 March 2016 3,006 4 View

How can I do a reaction between polyamine and lipoic acid NHS ester in an organic solvent?

I want to do this reaction but as far as learned from literature, NHS ester-amine reaction is done in a phosphate buffer solution between pH 8 to 9. However, lipoic acid modified polymer...

27 June 2015 1,731 3 View

An economic method for the fluorescent labeling of PCR fragments?

I have a trouble with my pcr reactions and i want to ask you where is my fault.I designed six primer pairs according to Schuelke's M13 methods but I did not get any reaction.One of my primers...

23 December 2014 4,623 5 View

What is the reason for different fold changes in gene expression from independent experimental repeats in Real-time PCR experiments ?

25 October 2014 7,228 3 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View