Size-exclusion chromatography for the isolation of EVs?

Dear all, I am currently doing research on extracellular vesicles (EVs). I'd like to improve the isolation method by trying out size-exclusion chromatography (SEC) compared to serial...

07 September 2020 4,412 5 View

You can support me in a study on COVID-19?

Hello, I am a psychological researcher. We are conducting an investigation on COVID-19 and I hope to have your support....

17 July 2020 8,402 13 View

Which is the optimal optical density for toxical recombinant protein expression in E.coli BL21 (AI)?

Hi everyone. I am trying to express and purify a recombinant antimicrobial peptide using as a host cell E. coli BL21 (AI) (employing L-arabinose and IPTG). Because this peptide can be toxic to...

19 February 2020 4,888 4 View

How reliable are third-party pollsters such as SurveyMonkey, Qualtrics and QuestionPro for your thesis?

I do understand they are being used in consumer or market research. But what about if you engage them for your thesis? Are they reliable? Because of time constraints and for convenience, some...

13 December 2019 493 3 View

Is it reasonable to perform Free Energy Landscape (FEL) anallysis on molecular dynamics simulations of 50ns?

Hello everyone, I'm having an issue with a review of a paper. I performed 50ns MD simulations on docking poses to know if the predicted poses were stable. Among the literature doing similar...

24 October 2019 4,608 5 View

Can LiCl precipitation be used in purifying low RNA concentration (30ug/ml) ?

RNA which has been extracted from blood collected in Heparin tubes are not showing any amplification after cDNA synthesis, in comparison to Blood of same patient collected in ETDA tubes. Any...

25 September 2019 9,026 2 View

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07 August 2019 2,592 2 View

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04 August 2019 5,642 2 View

I have received a ginger extract, and it is written concentration 25%, and it is one liter. so what does it mean?

My research is to study the ginger effect on breast cancer cells. so how to know the best concentration to treat the cells with it to study the effect. Note that i will treat it with different...

16 February 2019 4,419 3 View

Is there a survey I can use that asks people to rate behaviors?

I am currently looking for an existing survey that will help me ask students how they view behavior. I am thinking that I can ask them to rate how appropriate a situation is (e.g. crying in...

22 October 2018 9,145 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View