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Questions related from Thiyagarajan Sekar
Lox p was integrated at correct location, but unable to express cre recombinase to delete gene. Performed at different log hour and different Galactose concentration. Even though no gene deletion...
18 June 2024 967 3 View
Hi all, Earlier I got the recombinant protein expression in E. coli. Now, I repeat the expression from glycerol stock but i didnt get the protein expression. I repeat the fresh clone and...
10 December 2017 1,383 2 View
Hi, I am working in recombinant protein expression in E. coli BL21(DE3). Earlier I got the over-expressed proteins at 70 kDa. And I stored my construct as glycerol stock in -80 C. Now, I am...
04 November 2017 4,837 2 View
