Why myoblast cells do not differentiate by adding 2% Horse serum?

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Abdul Mannan Baig

Have you tried FBS?

Pengpeng Bi

Lower passage number is important for a good differentiation. Always pass the cell before 50% confluent.

Dragos Peptanariu

Try adding also insulin (Sigma-Aldrich #I-6634) to a final concentration of 1uM. Change differentiation medium every 24 hours for the first 3 days and every 12 hours in the next days.

Ishmael Mohammadi

No, just was used from horse serum.

I used the passage 30 and a 90% confluent .