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Questions related from Bahar Ksh
I'm trying to investigate cell viability after inhibiting a gene and then treating the cells with a drug. The viabilities I get are not usually in order. For example (in percentage): 105, 98, 81,...
14 March 2025 4,490 12 View
I am doing MTT assays with two adherent cell lines. After a few adjustments, one cell line is all set up and the variation between technical repeats (3 wells) in a plate is minimal. But for the...
21 January 2025 1,921 7 View
Hi Everyone, I'm trying to transfect ear cell lines with gene-specific siRNA and also expression plasmid, and then treat them with a drug to see the difference in response. Transfecting 24h before...
10 September 2024 2,709 2 View
I was performing an MTT assay to check cell viability. Due to a technical issue in the plate reader I was using, I had to read the plate at 540 nm wavelength. Would the results still be credible?
18 August 2024 6,353 5 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
24 July 2024 2,813 6 View
I'm going to transfect cells with expression plasmids and later siRNAs against the same gene. Is it true that penicillin/streptomycin in the media negatively affects these processes? Shout I use...
08 July 2024 8,788 6 View
I'm working with HEI-OC1 cells of the inner ear. Recently I'm seeing these round particles in the flask (picture attached), but cells' morphology and growth seems normal. However, there are...
27 June 2024 8,977 7 View
