Bahar Ksh

5 Questions 12 Answers 0 Followers

Questions related from Bahar Ksh

Can cells be transfected and treated with drug at the same time?

Hi Everyone, I'm trying to transfect ear cell lines with gene-specific siRNA and also expression plasmid, and then treat them with a drug to see the difference in response. Transfecting 24h before...

10 September 2024 2,704 2 View

What wavelength is suitable for MTT assay?

I was performing an MTT assay to check cell viability. Due to a technical issue in the plate reader I was using, I had to read the plate at 540 nm wavelength. Would the results still be credible?

18 August 2024 6,344 5 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

24 July 2024 2,792 6 View

Is antibiotic bad for transfection?

I'm going to transfect cells with expression plasmids and later siRNAs against the same gene. Is it true that penicillin/streptomycin in the media negatively affects these processes? Shout I use...

08 July 2024 8,776 6 View

What are these round particles in cell culture flask?

I'm working with HEI-OC1 cells of the inner ear. Recently I'm seeing these round particles in the flask (picture attached), but cells' morphology and growth seems normal. However, there are...

27 June 2024 8,959 7 View