I need to culture human granulosa cells from ivf patients. I use DMEM + F12 with 15% FBS, but cells did not grow.
Has anyone successfully cultured these cells?
Thank you so much for your help.
Hi,
Try to use the following medium composition: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS), Antibiotic.
Good luck!
Think natural, add 5mM estrogen and see them go multiplying.
Best,
Mannan
HI
below link gives the protocol for culture granulosa cells from ivf patient.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3370046/
hope this helps u.
Actually, my question is: can the transcription factor bind to RNA? Do you know a tool for finding transcription factors that bind to RNA?
14 October 2020 3,919 3 View
today, sts and thier parents think that they cant learn thorough media such as wahtsapp how to improve students' motivation through watsapp.
23 September 2020 9,756 5 View
I want to see the format of formulation in your code, so i can write for my own problem. U, DUDT, DUDG(NTGRD), FLUX(NTGRD), DFDT(NTGRD), DFDG(NTGRD,NTGRD)
04 August 2020 5,268 4 View
I designed a multi-junction solar cell in the Silveco Atla, which has two tunnel regions. But when I use a quantum mesh for these regions, my simulation is having trouble. Can anyone guide me?
04 May 2020 2,121 3 View
The results showed low expression of A and overexpression of B in Tumor tissues. We expect them to show a negative correlation, but they show a significant positive correlation. Can you explain...
06 March 2020 3,149 11 View
Dear all, I am going to measure the amount of drug degradation after release of nanoparticles in the release medium. Due to gradual release of the drug, the drug concentration will be increased...
09 January 2020 8,841 2 View
It takes 1 min to translate everything but it takes 25 years to know if it has translated correctly. I had a client, a pharmacy graduate, called Dr. Grand (for ethic's sake, I'm gonna call her...
29 November 2019 3,603 8 View
I want to delete some solvent molecules in box after md production, but I don't the lables of them and how delete them and how I delete a specific molecule??
11 November 2019 6,415 1 View
I'm studying hormonal adulterant in the dietary supplement. At the beginning of validation, I inject the concentration series of hormone standards. But I had not repeatable result in an injection...
05 October 2019 8,695 2 View
hi i'm going to do a reaction between Carboxylic acids and amine. is there any body who can introduce some papers or something to find out that we need how much of each component ?? and what is...
16 September 2019 8,176 8 View
02 March 2021 3,060 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....
28 February 2021 7,967 3 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
28 February 2021 9,936 2 View
The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...
27 February 2021 6,350 2 View