Why my Granulosa cells are not growing in media?

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Justyna Augustyniak

Hi,

Try to use the following medium composition: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS), Antibiotic.

Good luck!

Abdul Mannan Baig

Think natural, add 5mM estrogen and see them go multiplying.

Best,

Mannan

Sreedhar V

below link gives the protocol for culture granulosa cells from ivf patient.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3370046/

hope this helps u.