Robert Adolf Brinzer
Grow the plant in agar and use fluorescence to quantify the infections.
This paper may be useful if you want to automate the process (Article From root to shoot; Quantifying nematode tolerance in Arabid...
).
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Hari Gaur
The simplest method is to grow the plant with the given seed to be tested; inoculate the root zone with about 100 freshly hatched second-stage juveniles (J2) per Kg soil; remove the roots carefully after 4 weeks, keep them in a warm place and observe for root galling and production of egg sacs with eggs. If the root galling is absent or less than 10-20 galls are present depending on the size of the root system; Observe the egg sacs on root and the number of eggs per egg sac. As a final step, soil samples can be processed to extract J2 from soil, and count. Calculate the reproduction factor by dividing the final number of J2 by inoculation level. If the number of galls is low and the reproduction factor is less than 2, the variety can said to be resistant. The root-galling can also be classified on a 0-5 scale based on the number of galls or percentage of root system galled. Root-knot index lesser than 2 may be considered resistant (not immune).
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