my agrobacterium not grow in abk(Agrobacterium Minimal Medium with kanamicin).
we got colony from lbk medium. it grow well. but when we do abk, agro never grow...
what is the problem?
Lee Si Yeon Most of Agrobacterium tumefaciens strains will grow on Abk, but A. rhizogenes, A. rubi, some other natural groups of strains are not prototrophic, and will need other sources of N and growth additives. You also may have an auxotrophic mutant...
Citing - "Depending on the strain and the composition of plate medium, it will take 2–4 days for colonies to appear at 25°C. The growth of even prototrophic strains of Agrobacterium will proceed faster when amino acids and vitamins are supplied..." (A.A. Wise et al. Culture and maintenance of Agrobacterium strains. In: Methods in Molecular Biology. Vol. 343.)
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