Dear Dong Ding

HEK293T is a kidney cell line.

As you mentioned the TBG promoter is liver specific, so this might explain why you are not be able to detect expression of transgene from the AAV8 vector carrying TBG promoter.

Yes, you can use an AAV2/8 particle without TBG promoter. For examples, AAV-mediated expression in HEK293T cells can be achieved by a ubiquitous promoter such as CMV.

Alternatively you can use a liver cell lines to test AAV2/8 vector with TBG promoter. Pls check options of hepatic cell lines - Article Immortalized Human Hepatic Cell Lines for In Vitro Testing a...

However, keep in mind that different serotypes have different tropism. This recent review might be useful - https://www.cell.com/trends/molecular-medicine/fulltext/S1471-4914(20)30257-4

AAV8 is better for transduction in vivo compared to in vitro.

So for in vitro transduction you might need to use higher MOIs or use arsenic oxide to enhance transduction (https://www.cell.com/action/showFullTableHTML?isHtml=true&tableId=t0010&pii=S1471-4914%2820%2930257-4)

Hope this helps !

Could you use the anti-AAV monoclonal antibody to detect the expression?

https://humimmu.com/products/by-products-type/aav-antibodies/anti-aav8-antibody.html

https://humimmu.com/products/by-products-type/aav-antibodies/anti-aav9-antibody.html

https://humimmu.com/products/by-products-type/aav-antibodies/anti-aavhu37-antibody-clone-hi3701.html

It works in our assay.