I am using two microalgae strains (Haematococcus pluvialis and Chlorella zofingiensis) which are well known for production of astaxanthin. but after 15-20 days growth of microalgae in TAP medium the coloration of medium is changed to yellow and after analysis in the HPLC the lutein concentration is high as compared to astaxanthin. Is there any possible reason behind synthesis of lutein?
It's possible that this is due to incomplete carotenogenesis. In reality, the major pigments in green algal cells are chlorophyll a, chlorophyll b, and lutein. When carotenogenesis begins, chlorophyll and lutein breakdown begins. It is possible that you should focus on stressor optimization in the second phase of the cultivation procedure.
Lutein is one of the main xanthophylls involved in photosynthesis. Astaxanthin, on the other hand, is a sunscreen carotenoid without any photosynthetic functions and never even accumulated in chloroplasts. You may suggest further answer yourself.
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