Hi
I am working on liposome formulation. we are trying for reproduction of the size at range of +or- 20 nm. but Malvern zeta Sizer always showing different size with 2-3 peaks always.
There are number of publications just mentioned the size in number like ZXC +/- AB.
really frustrated with this first problem
and the second problem is losing hope to trust literature publications.
please share your experience, guide me if possible. help me please.
Thank you.
JR
Hi Raj,
It sounds like your sample has some problem. This can be the case if too polydisperse, liposomes not stable, too big, concentration too low, and the list goes on. You can’t just trust a publication. If you’re following their protocol exactly then I’d recommend to contact the authors, try troubleshooting yourself, or if that doesn’t work then you can ask a company to do the work for you. I recommend LipExoGen who always does an excellent job on liposome preps for our lab and save us a lot of time. Getting the particle size in the range of +/- 20 nm is no problem for them.
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