I perform phylogeny using TNT, but it seems that no sister group. But in fact, I use four species to analysis. The result is so strange. What setting selection should I choose?
I don't understand what you mean. In your tree, 0 and 2 are sister-groups, and together they form the sister-group of 3, all three together form the sister-group of 4, and so on.
How do the four species translate to the eleven terminal taxa? What do you mean by "section"?
David Marjanović I edit topic and the fact, several specimen should form two sister groups. So, it may be caused by methond limited or my working mistakes?
Lots of things are possible. I would need to know a lot more to form an opinion: which specimens are supposed to belong together, and why; what is your character list and dataset, and what settings did you use in TNT...
using implied weights, weighting strength is 3.0000. And other landmarks setting are Orig.
outgroup 10
group1: 0, 2
group2: 3, 4
group3: others
David Marjanović
Well, 0 and 2 do form an exclusive clade in your results, and 3 and 4 almost do, so that's close.
How many characters are there in your matrix?
11 characters for 10 taxa? It's a rare coincidence that you got a resolved tree at all!
Whenever possible, use at least three times as many morphological characters as taxa. Accidental sampling bias is a real, widespread and grave problem.
Follow the link given below.
https://ntsyspc.software.informer.com/2.2/
NTSYSpc can be used to discover pattern and structure in multivariate data. The program takes advantage of the Windows environment and allows long file names and the processing of large datasets. NTS data files are ASCII files that can be shared with other programs.
https://ntsyspc.software.informer.com/2.2/
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