During my experiment on the synthesis of selenium nanoparticles (SeNPs) using leaf extract (distilled water extraction) I observed that when I used a 1:9 ratio (1 part extract and 9 parts of 0.1M sodium selenite), a red color was obtained. However, when I attempted to scale up the synthesis using a higher concentration ratio, such as 10:90, I did not achieve the desired result. Additionally, even when I tried a 5:5, and 1:3 ratio, the red color was still not observed.