Hi there,

The only advantage of Y258 over BY4741 is that this strain is deficient for PEP4 which is the major protease of S. cerevisiae. Therefore protein degradation is reduced in Y258. But one can consider performing protein expression in BY4741 if Y258 is not available in your lab.

Dear Dominique,

Thanks a lot for your answer. It is indeed a great help to me. I have no experience in protein expression in yeast. I have only worked with bacterial RPP expression.

Kind regards,

Charan

Hi Charandeep,

Same comment here, we use in our lab many different yeast strains, engineered ot not, for expression of a large number of recombinant proteins : none of them is Y258 !!! ALl others work quite fine.

Best regards,

Philippe

Dear Philippe,

Thanks a lot for your answer. Now, I am very motivated to follow on with protein expression in standard yeast strain we have in our lab.

Kind regards,

Charan

Dear N.James,

I am not sure. I don't use yeast often for protein expression. My personal preference is to use bacterial strains.

Kind regards,

Charan