Is research on antimicrobial peptides worth it?

In the age of Mutiresistant drug bacterium(s) and eukaryotic pathogenic organisms or superbugs, we are in a constant lookout for new treatments and especially antibiotic drugs. Two approaches, in...

23 August 2020 3,915 3 View

Is ATP the best energy providing molecule? can there be something better?

ATP energy-storing molecule as is no doubt an efficient! as not only it is used as energy storage and provider in all forms of life and has probably been around for billions of years. Even in the...

08 February 2020 9,015 6 View

How to dissolve Ethylene Vinyl Acetate?

I need to get rid of the ethylene vinyl acetate paste stuck onto a glass surface. What would be the best solvent to get rid of it?

09 August 2019 9,016 6 View

Is there any good pub-med journal of otorhinolaryngology which publishes images of interesting cases ?

Certain individual cases are very interesting and the experience of managing could be shared through images with brief detail.

18 September 2018 7,651 3 View

What are dose and exposure time for cancer radiation therapy? Please, give some ideas or papers. Thanks?

Cancer radiation therapy.

11 September 2018 7,623 5 View

In Abaqus assembly, if there are repeated parts but different orientations is there a procedure to mesh/include geometric correction in common?

I have a assembly with around 300 parts. If a few parts are similar but their orientation differs is there a procedure to automate the meshing of all these parts.

22 April 2018 6,378 1 View

Children are the most vulnerable and voiceless in our society. What are the major causes of child abuse and how can we let their voices be heard?

Children are the most vulnerable and voiceless in our society. How can we address the issue of child abuse and let their voices be heard? Should a child leave home being a victim of extreme violence?

07 April 2018 2,014 4 View

No title

Do both are affected by initial glucose concentration in media ? I have taken initial glucose (substrate) in following concentrations (g/l) : 20, 40, 60, 80, 100. Rest other components of media...

05 April 2018 9,279 4 View

Why double band appeared on TLC plate?

I run standard mixture on TLC plate. All standards showed with double band.

26 February 2018 3,292 3 View

What is the differences between gene silencing by shRNA and miRNA? What are procedural and theoretical differences?

I want to know advantages and disadvantages of gene silencing by shRNA and miRNA?

10 February 2018 8,861 3 View

Can anyone suggest a commercial MAT kit to detect Toxoplasma gondii antibodies in animals?

18 January 2021 5,438 2 View

Does anyone grow Mycobacteria in TECAN Spark machine to get growth curves? What setting is necessary? Any tips (use Tween20 or tyloxapol?)?

I would like to grow Mycobact. Smegmatis in 24well plate in TECAN Spark machine. So far, I was using 100ml erlenmeyer flasks with 20 ml bacterial culture w/ shaking 180 rpm in 37C . Tecan would...

10 January 2021 6,624 2 View

Is there any cheap spacer on nucleotide (other than photocleavable) that can be cut off chemically?

07 December 2020 1,730 2 View

What does a higher LoD indicate in context of COVID19?

I have seen COVID19 test LoD ranging from 180 to 600000 NAAT Detectable Units/mL So a rapid antigen test with LoD of TCID50 1.13 x10^2 per mL and RT PCR TCID50 1x10^-2 per mL (600 NDU/mL) and a...

28 November 2020 1,238 3 View

Can I get exosomes from cryopreserved whole blood supplemented with concentration of DMSO (10%)?

I read many papers about exosome isolation from fresh blood or frozen plasma, but not for frozen whole blood. I am wondering whether can I get exosomes from cryopreserved whole blood? One of my...

26 October 2020 5,166 3 View

Do you know any diagnosis that is based on fluorescence intensity of marked cells ?

I am a physicist, so I know the techn not the biological applications. The question is straightforward. I am looking for a diagnosis that rely on fluorescent based cell imaging where the intensity...

25 October 2020 1,653 3 View

What assays / application would benefit the most from absolute quantitative fluorescence measurement ?

Many assays, including Cell-based assays, QPCR, some ELISA test, rely on the presence/absence of fluorescence. Diagnosis applications about cancer or antiobiotic resistance uses fluorescence ratio...

21 October 2020 1,159 3 View

Is there a possibility that nontuberculous mycobacteria (NTM) can be isolated in public playgrounds?

Since the nontuberculous mycobacteria (NTM) are environmental pathogens in which they are found in water, soil, and dust, is there a possibility that NTMs can be isolated in public places or...

06 October 2020 4,878 6 View

MiRNAs from exosome?

14 July 2020 9,371 6 View

Can we still rely of diagnostic tools such as the Well's score during the COVID19 epidemic?

The Well's score was designed to help predict the likelihood of PE by studying the PPV of PE of various clinical features in a population; obviously that population was not one in which COVID19...

10 May 2020 2,073 5 View